Journal: Frontiers in Microbiology
Article Title: Megasphaera elsdenii Lactate Degradation Pattern Shifts in Rumen Acidosis Models
doi: 10.3389/fmicb.2019.00162
Figure Lengend Snippet: Substrate lactate abundance and pH can regulate M. elsdenii lactate degradation pattern in continuous culture. (A) Growth curves of M. elsdenii in medium under different conditions. (B) The mean proportions of acetate, propionate and butyrate in different groups. The area of the circle corresponds with the percentage of this acid. White background represents the pH of 5.5 while blue means pH of 6.5. (C) M. elsdenii lactate degradation pathways and relative expression of genes involved in metabolic pathway. Pink box means pH at 5.5 while green box represents pH at 6.6. ARC, acrylyl-CoA reductase; MCD, methylmalonyl-CoA decarboxylase; MCM, methylmalonyl-CoA mutase; SCS, succinyl-CoA synthetase (succinyl thiokinase); FR, fumarate reductase; FH, fumarase (fumarate hydratase); MD, malate dehydrogenase; PC, pyruvate carboxylase; PD, pyruvate dehydrogenase; PCT, propionyl-CoA transferase; LCD, lactyl-CoA dehydratase; LR, lactate racemase; D-LDH, D-lactate dehydrogenase; L-LDH, lactate dehydrogenase; PTP, phosphate propionyl transferase; PRK, propionate kinase; PTB, phosphate butyryl transferase; BUK, butyrate kinase; PTA, phosphate acetyl transferase; ACK, acetate kinase; BCD, butyryl-CoA dehydrogenase.
Article Snippet: Four lactate producers S. bovis (CCTCC AB2016240, produces lactate mainly and also formate and acetate) , L. fermentum (ATCC 11976, produces lactate), B. fibrisolvens (ATCC 27208, produces lactate, formate, and acetate) , S. ruminantium (ATCC 12561, lactate mainly and also formate, acetate, and butyrate) ( ) and one lactate utilizer M. elsdenii (ATCC 25940, can produce acetate, propionate, and butyrate) ( ) were used in this study.
Techniques: Expressing